Composite

Part:BBa_K5097016:Design

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-25)


sBFP with 6X His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 91


Design Notes

Removed native stop codon and added an AGVG linker and 6x His tag. A double-stop codon was added at the end.


Source

BBa_K156010

References